eBIOSENSE Project

The aim of the eBIOSENSE project is to develop a method for rapid detection of pathogenic microorganisms by means of electrical biochips. The aim of our group is to detect Escherichia coli strains producing Shiga toxins, derivatives of the strain O157:H7. The detection will be based on identification of DNA sequences corresponding to genes coding for these toxins (stx1 and stx2), a gene coding for the O157 antigen as well as toxins and antigens themselves. The stx genes are carried by lambdoid bacteriophages, thus we also try to detect E. coli O157:H7 by the use of specific phage antigens.

Project participants

Supervisor

Prof. Grzegorz Węgrzyn

e-mail:  wegrzyn@biotech.ug.gda.pl

phone: +4858 346 30 14

Executors

Dr Marcin Łoś

e-mail:  mlos@biotech.ug.gda.pl

phone: +4858 523 63 19

Dr Joanna Łoś

e-mail:  jlos@biotech.ug.gda.pl

phone: +4858 523 63 19

Publication:

Marcin Łoś, Joanna Łoś, Lars Blohm, Edzard Spillner, Thomas Grunwald, Joerg Albers, Rainer Hintsche, Grzegorz Węgrzyn (2005) Rapid detection of viruses using electrical biochips and anti-virion sera. Letters in Applied Microbiology, 40, 479-485

Magdalena Gabig-Cimińska, Anders Holmgren, H. Andresen, Kim Bundvig Barken, Mogens Wumpelmann, Jorg Albers, Rainer Hintsche, Antje Breitenstein, Peter Neubauer, Marcin Łoś, Agata Czyż, Grzegorz Węgrzyn, Gustav Silfversparre, Britta Jurgen, Thomas Schweder, Sven-Olof Enfors (2004) Electric chips for rapid detection and quantification of nucleic acids. Biosensors and Bioelectronics, 19, 537-546

Magdalena Gabig-Cimińska, Marcin Łoś, Anders Holmgren, Jörg Albers, Agata Czyż, Reiner Hintsche, Grzegorz Węgrzyn, Sven-Olof Enfors (2004) Detection of bacteriophage infection and prophage induction in bacterial cultures by means of electric DNA chips. Analytical Biochemistry, 324, 84-91